Upgrade StorageImporting RIS: "the selected file is not in a supported format"
I am importing files from Ovid that are RIS. I can open them in Endnote no problem. For some reason they are giving me file is not supported error in Zotero. I have checked and the file starts with TY and ends in ER as it is supposed to. Below is the first record.
TY - JOUR
DB - Ovid MEDLINE(R) Epub Ahead of Print
ID - 41435780
T1 - Simultaneous chiral analysis of DL-asp and DL-alpha-HB with derivatization in human fingernail by UHPLC-MS/MS: an application in colorectal cancer.
DO - https://dx.doi.org/10.1016/j.jchromb.2025.124895
A1 - Xu, Yinshuo
A1 - Shan, Yuanjie
A1 - Nishio, Yuta
A1 - Todoroki, Kenichiro
A1 - Toyo'oka, Toshimasa
A1 - Jin, Toufeng
A1 - Min, Jun Zhe
Y1 - 2025//
Y2 - 20251218//
N2 - With the development of untargeted metabolomics, studies have shown that alterations in the serum levels of specific amino acids are strongly correlated with the development of early-stage colorectal cancer (CRC). However, the relationship between optically active free amino acids in human nails and the risk of CRC remains unclear. In this study, a highly sensitive UHPLC-MS/MS chiral separation method based on (S)-(-)-DBD-Pro-COCl derivatization was established for the simultaneous detection of DL-Asp, DL-Kyn, DL-alpha-hydroxybutyric acid (DL-alpha-HB), and Cystamine in the fingernails of patients with CRC. The derivatization reagents were incubated with DL-amino acids, DL-alpha-HB and Cyst at 60 degreeC for 15 min to form diastereomers. Subsequently, an Imtakt cadenza CD-C18 column (3.0 x 150 mm, 3.0 mcm) was used for the separation of diastereomers under gradient elution of 10 mM HCOONH4 in H2O as mobile phase A and 0.1 % HCOOH in CH3CN as mobile phase B. The degrees of separation (Rs) ranged from 1.76 to 3.21. R2 was >0.9995, indicating good linearity; the limit of detection (LOD) was 25-100 fmol; the interday and intraday precision of DL-Asp and DL-alpha-HB detection were 0.85 %-4.91 %; and the average recovery ranged from 81.12 % to 110.2 %. However, during the determination process of fingernail samples, only DL-Asp and DL-alpha-HB were successfully detected, while DL-Kyn and Cyst were not detected. Therefore, this novel method was used to quantify DL-Asp and DL-alpha-HB in fingernail samples from 32 healthy volunteers (HVs) and 16 patients with CRC (CPs). The results revealed significant differences in D-Asp (p < 0.05), L-Asp (p < 0.01), and L-alpha-HB (p < 0.05) levels between male HVs and CPs and in D-alpha-HB (p < 0.05) levels between female HVs and CPs. In addition, the D/L-alpha-HB ratio (p < 0.01) was significantly different between male HVs and CPs. These findings validate the use of DL-amino acids, DL-alpha-HB and Cyst from human fingernails as potential diagnostic biomarkers for CRC, providing a basis for the clinical application of human fingernail samples for the noninvasive diagnosis of CRC. Copyright © 2025 Elsevier B.V. All rights reserved.
JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
JA - J Chromatogr B Analyt Technol Biomed Life Sci
VL - 1270
SP - 124895
CY - Netherlands
M2 - Xu, Yinshuo. Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Department of General Surgery and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China.
M2 - Shan, Yuanjie. Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Department of General Surgery and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China.
M2 - Nishio, Yuta. Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
M2 - Todoroki, Kenichiro. Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
M2 - Toyo'oka, Toshimasa. Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
M2 - Jin, Toufeng. Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Department of General Surgery and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China. Electronic address: tfjin@ybu.edu.cn.
M2 - Min, Jun Zhe. Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Department of General Surgery and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China. Electronic address: junzhemin23@163.com.
SN - 1873-376X
SN - 1570-0232
M1 - 101139554
PT - Journal Article
L2 - https://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=reference&D=medp&NEWS=N&AN=41435780
ER -
TY - JOUR
DB - Ovid MEDLINE(R) Epub Ahead of Print
ID - 41435780
T1 - Simultaneous chiral analysis of DL-asp and DL-alpha-HB with derivatization in human fingernail by UHPLC-MS/MS: an application in colorectal cancer.
DO - https://dx.doi.org/10.1016/j.jchromb.2025.124895
A1 - Xu, Yinshuo
A1 - Shan, Yuanjie
A1 - Nishio, Yuta
A1 - Todoroki, Kenichiro
A1 - Toyo'oka, Toshimasa
A1 - Jin, Toufeng
A1 - Min, Jun Zhe
Y1 - 2025//
Y2 - 20251218//
N2 - With the development of untargeted metabolomics, studies have shown that alterations in the serum levels of specific amino acids are strongly correlated with the development of early-stage colorectal cancer (CRC). However, the relationship between optically active free amino acids in human nails and the risk of CRC remains unclear. In this study, a highly sensitive UHPLC-MS/MS chiral separation method based on (S)-(-)-DBD-Pro-COCl derivatization was established for the simultaneous detection of DL-Asp, DL-Kyn, DL-alpha-hydroxybutyric acid (DL-alpha-HB), and Cystamine in the fingernails of patients with CRC. The derivatization reagents were incubated with DL-amino acids, DL-alpha-HB and Cyst at 60 degreeC for 15 min to form diastereomers. Subsequently, an Imtakt cadenza CD-C18 column (3.0 x 150 mm, 3.0 mcm) was used for the separation of diastereomers under gradient elution of 10 mM HCOONH4 in H2O as mobile phase A and 0.1 % HCOOH in CH3CN as mobile phase B. The degrees of separation (Rs) ranged from 1.76 to 3.21. R2 was >0.9995, indicating good linearity; the limit of detection (LOD) was 25-100 fmol; the interday and intraday precision of DL-Asp and DL-alpha-HB detection were 0.85 %-4.91 %; and the average recovery ranged from 81.12 % to 110.2 %. However, during the determination process of fingernail samples, only DL-Asp and DL-alpha-HB were successfully detected, while DL-Kyn and Cyst were not detected. Therefore, this novel method was used to quantify DL-Asp and DL-alpha-HB in fingernail samples from 32 healthy volunteers (HVs) and 16 patients with CRC (CPs). The results revealed significant differences in D-Asp (p < 0.05), L-Asp (p < 0.01), and L-alpha-HB (p < 0.05) levels between male HVs and CPs and in D-alpha-HB (p < 0.05) levels between female HVs and CPs. In addition, the D/L-alpha-HB ratio (p < 0.01) was significantly different between male HVs and CPs. These findings validate the use of DL-amino acids, DL-alpha-HB and Cyst from human fingernails as potential diagnostic biomarkers for CRC, providing a basis for the clinical application of human fingernail samples for the noninvasive diagnosis of CRC. Copyright © 2025 Elsevier B.V. All rights reserved.
JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
JA - J Chromatogr B Analyt Technol Biomed Life Sci
VL - 1270
SP - 124895
CY - Netherlands
M2 - Xu, Yinshuo. Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Department of General Surgery and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China.
M2 - Shan, Yuanjie. Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Department of General Surgery and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China.
M2 - Nishio, Yuta. Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
M2 - Todoroki, Kenichiro. Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
M2 - Toyo'oka, Toshimasa. Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
M2 - Jin, Toufeng. Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Department of General Surgery and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China. Electronic address: tfjin@ybu.edu.cn.
M2 - Min, Jun Zhe. Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Department of General Surgery and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China. Electronic address: junzhemin23@163.com.
SN - 1873-376X
SN - 1570-0232
M1 - 101139554
PT - Journal Article
L2 - https://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=reference&D=medp&NEWS=N&AN=41435780
ER -
If the entry above does work, you'll need to cut down the file in halves until you find the entry that's invalid.